ap2α  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: AAV2-Retro-Mediated Gene Transfer Selectively Targets Outer Retinal Cells Following Intravitreal Injection

doi: 10.64898/2026.03.10.710806

Figure Lengend Snippet: (A) Stitched 10X images of retinal cross-sections showing mGL expression at 14 dpi. (B) Higher-magnification view of a representative retinal region with mGL expression. (B’) Merged image from (B) highlighting layer-specific distribution (RPE, ONL, OPL, INL, IPL, and GCL), mGL, and DAPI across the layers. (C) Retinal flat-mount at 14 dpi after sequential injections, showing widespread mGL expression. (C’ and C’’) Higher magnification images of the boxed areas in (C). Scale bars: 200 µm (A, C); 50 µm (B’, C’, C’’). (D) Quantification of mGL- positive cells within the regions of high transduction in the ONL (i.e., region of interest, ROI). The percentage shown indicates the proportion of transduced cells among total DAPI-positive ONL cells. N= 3 animals. Scale bars : 200 µm (A, C); 50 µm (B’, D’).

Article Snippet: Samples were then incubated with anti-RBPMS antibody (1:2,000; Custom made at ProSci), anti-Cone arrestin (1:200; cat# AB15282; Sigma Aldrich), and anti- AP-2α antibody (1:50; cat# 3B5; DSHB) that were diluted in PBST blocking solution and incubated at 4 °C overnight.

Techniques: Expressing, Transduction

Journal: bioRxiv

Article Title: AAV2-Retro-Mediated Gene Transfer Selectively Targets Outer Retinal Cells Following Intravitreal Injection

doi: 10.64898/2026.03.10.710806

Figure Lengend Snippet: Retinal cross-sections show mGL expression (green) at 3 dpi (A) and 14 dpi (B). AP-2α immunoreactivity shown in magenta and RBPMS immunoreactivity in red. (A’ and B’) Higher-magnification images showing cells which appear to be co-labelled with mGL and AP-2α. INL, inner nuclear layer; GCL, ganglion cell layer. (C) Quantification shows that the percentage of mGL + cells that are positive for either RBPMS (in GCL and INL) or AP-2α at each time point. N=3 per group. Error bar, ± SEM Two-way ANOVA with Tukey’s multiple comparisons: * < 0.05, **** < 0.0001, ns = not significant. Scale bars : 50 µm (A, B); 5 µm (A’, B’).

Article Snippet: Samples were then incubated with anti-RBPMS antibody (1:2,000; Custom made at ProSci), anti-Cone arrestin (1:200; cat# AB15282; Sigma Aldrich), and anti- AP-2α antibody (1:50; cat# 3B5; DSHB) that were diluted in PBST blocking solution and incubated at 4 °C overnight.

Techniques: Expressing